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Assay Method Information

Assay Name:  DXR Inhibition Assay for Fosmidomycin
Description:  Assays were performed in 50 mM Tris/HCl buffer, pH 7.5, containing 3 mM MgCl2 and 2 mM DTT. The concentrations of NADPH and DXP were 0.15 and 0.5 mM respectively. When the reaction was initiated by H-DXR, the inhibition concentrations varied from 0.1 to 0.5 ÁM for fosmidomycin. When the enzyme was pre-incubated with the inhibitor, concentrations from 0.01 to 0.15 ÁM for fosmidomycin were used. Eyzme assays were run under the same conditions as those described above. The concentration of inhibitors were varied from 0.05 to 0.5 ÁM for fosmidomycin. The progress of reaction was monitored at 340 nm over 5 min.
Affinity data for this assay
 

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Last update November 1, 2007
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