BindingDB logo
myBDB logout

Assay Method Information

Assay Name:  Fluorescence Binding Assays
Description:  Varying amounts of enzyme were diluted in PBS supplemented with 0.5 g/L pluronic F127 and incubated with 500 nM ML349-FL for 30 minutes at room temperature. The fluorescent polarization from each well was measured using a Tecan F500 plate reader using 485/20 nm excitation and 535/25 nm emission polarization filters. Each concentration was measured in 2 replicates.
Affinity data for this assay
 

If you find an error in this entry please send us an E-mail
   
    

Home

|

Search

|

Deposit

|

SiteMap

|

About us

|

Email us

|

Info

 
Last update November 1, 2007
©2000 BindingDB. All rights reserved.