| Assay Method Information | |
| | Mammalian Two-Hybrid Assay |
| Description: | We performed a mammalian one-hybrid assay in HeLa cells, where we transfected a construct coding for a Gal4DBD-BagN128 fusion or just Gal4DBD alone togetherwith a Gal4 binding site-luciferase reporter gene and an AR expression vector. The transfected cells were then treated with or without DHT, and the reporter gene activity was measured. |
| Affinity data for this assay | |
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