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Assay Method Information

Assay Name:  Slow-Binding Inhibition Assay
Description:  Progress curves for slow-binding inhibition analysis were obtained by adding nitrocefin (final concentration 36 μM) to a solution containing enzyme (0.13 μg/mL) and varying concentrations of the inhibitor thiomaltol in buffer (50 mm MOPS at pH 7.0; final volume 1 mL) in acrylic cuvettes. Hydrolysis of the substrate was monitored for up to 3 min.
Affinity data for this assay
 

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Last update November 1, 2007
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