Assay Method Information | |
| ChEMBL_1580602 (CHEMBL3810610) |
Description: | Mixed type inhibition of human recombinant full length GST-tagged CDC25A transfected in Escherichia coli BL21-DE3 assessed as enzyme/substrate/inhibitor complex using 3-O-methylfluorescein phosphate as substrate at 5 uM incubated for 20 mins followed by substrate addition measured every 10 mins for 2 hrs by fluorimetric assay |
Affinity data for this assay | |
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