| Assay Method Information | |
| | Cyclooxygenase activity assay |
| Description: | Reaction mixture containing 100 mM Tris-HCl buffer (pH 8) and COX-1 (ovine) or COX-2 (human recombinant) was preincubated for 10 min in a water bath at 37 °C. The reaction was initiated by the addition of 10 µL arachidonic acid (final concentration 100 µM). After 2 min, the reaction was terminated by adding 1 M HCl. Test compounds were dissolved in DMSO and diluted to the desired concentration with potassium phosphate buffer of pH 7.4. |
| Affinity data for this assay | |
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