BindingDB logo
myBDB logout

Assay Method Information

Assay Name:  DPPS Inhibition (trans FPP based) Assay
Description:  We screened an in-house library of 53 compounds using trans-FPP as substrate. Briefly, the condensation of IPP and GPP, FPP, or cis-FPP catalyzed by DPPS was monitored using a continuous spectrophotometric assay for diphosphate release in 96-well plates with 200 ÁL reaction mixtures containing 400 ÁM 2-amino-6-mercapto-7-methylpurine (MESG), 25 ÁM GPP, cis-FPP or trans-FPP, 200 ÁM IPP, 25 mM Tris-HCl (pH 7.5), 0.01% Triton-X-100, and 1 mM MgCl2.
Affinity data for this assay
 

If you find an error in this entry please send us an E-mail
   
    

Home

|

Search

|

Deposit

|

SiteMap

|

About us

|

Email us

|

Info

 
Last update November 1, 2007
©2000 BindingDB. All rights reserved.