BindingDB logo
myBDB logout

Assay Method Information

Assay Name:  A3 Adenosine Receptor Binding Assay
Description:  Aliquots of cell membranes (90 ug) were incubated at 25 °C for 180 min in 500 uL of binding buffer (50 mM Tris-HCl, 5 mM MgCl2, 1 mM EDTA, 2 units/mL ADA, pH 7.4) in the presence of 30 nm [3H]NECA and six different concentrations of the tested compounds. Non-specific binding was determined in the presence of 100 uM R-PIA.
Affinity data for this assay
 

If you find an error in this entry please send us an E-mail
   
    

Home

|

Search

|

Deposit

|

SiteMap

|

About us

|

Email us

|

Info

 
Last update November 1, 2007
©2000 BindingDB. All rights reserved.