Reaction Details Report a problem with these data
Target
Glutamate receptor ionotropic, NMDA 1 [F484A,T518L]/3B
Ligand
BDBM213889
Substrate
n/a
Meas. Tech.
Two-electrode Voltage Clamp Electrophysiology
pH
7.4±n/a
Temperature
298.15±n/a K
Ki
>1.50e+5±n/a nM
IC50
>3.00e+5±n/a nM
Comments
extracted
Citation
 Kvist, T; Steffensen, TB; Greenwood, JR; Mehrzad Tabrizi, F; Hansen, KB; Gajhede, M; Pickering, DS; Traynelis, SF; Kastrup, JS; Bräuner-Osborne, H Crystal structure and pharmacological characterization of a novel N-methyl-D-aspartate (NMDA) receptor antagonist at the GluN1 glycine binding site. J Biol Chem 288:33124-35 (2013) [PubMed]  Article 
Target
Name:
Glutamate receptor ionotropic, NMDA 1 [F484A,T518L]/3B
Synonyms:
GluN1(F484A/T518L)/N3B
Type:
Receptor
Mol. Mass.:
n/a
Description:
n/a
Components:
This complex has 2 components.
Component 1
Name:
Glutamate receptor ionotropic, NMDA 1 [F484A,T518L]
Synonyms:
Grin1 | NMDA receptor subunit GluN1-1a (GluN1 F484A/T518L) | NMDZ1_RAT | Nmdar1
Type:
Protein
Mol. Mass.:
105529.45
Organism:
Rattus norvegicus (Rat)
Description:
Rat NMDA receptor subunit GluN1-1a F484A/T518L mutant
Residue:
938
Sequence:
MSTMHLLTFALLFSCSFARAACDPKIVNIGAVLSTRKHEQMFREAVNQANKRHGSWKIQLNATSVTHKPNAIQMALSVCEDLISSQVYAILVSHPPTPNDHFTPTPVSYTAGFYRIPVLGLTTRMSIYSDKSIHLSFLRTVPPYSHQSSVWFEMMRVYNWNHIILLVSDDHEGRAAQKRLETLLEERESKAEKVLQFDPGTKNVTALLMEARELEARVIILSASEDDAATVYRAAAMLNMTGSGYVWLVGEREISGNALRYAPDGIIGLQLINGKNESAHISDAVGVVAQAVHELLEKENITDPPRGCVGNTNIWKTGPLFKRVLMSSKYADGVTGRVEFNEDGDRKFANYSIMNLQNRKLVQVGIYNGTHVIPNDRKIIWPGGETEKPRGYQMSTRLKIVTIHQEPFVYVKPTMSDGTCKEEFTVNGDPVKKVICTGPNDTFPGSPRHTVPQCCYGFCIDLLIKLARTMNFTYEVHLVADGKAGTQERVNNSNKKEWNGMMGELLSGQADMIVAPLLINNERAQYIEFSKPFKYQGLTILVKKEIPRSTLDSFMQPFQSTLWLLVGLSVHVVAVMLYLLDRFSPFGRFKVNSEEEEEDALTLSSAMWFSWGVLLNSGIGEGAPRSFSARILGMVWAGFAMIIVASYTANLAAFLVLDRPEERITGINDPRLRNPSDKFIYATVKQSSVDIYFRRQVELSTMYRHMEKHNYESAAEAIQAVRDNKLHAFIWDSAVLEFEASQKCDLVTTGELFFRSGFGIGMRKDSPWKQNVSLSILKSHENGFMEDLDKTWVRYQECDSRSNAPATLTFENMAGVFMLVAGGIVAGIFLIFIEIAYKRHKDARRKQMQLAFAAVNVWRKNLQDRKSGRAEPDPKKKATFRAITSTLASSFKRRRSSKDTSTGGGRGALQNQKDTVLPRRAIEREEGQLQLCSRHRES
  
Component 2
Name:
Glutamate receptor ionotropic, NMDA 3B
Synonyms:
Grin3b | NMD3B_RAT | NMDA receptor subunit 3B (GluN3B)
Type:
Protein
Mol. Mass.:
109185.76
Organism:
Rattus norvegicus (Rat)
Description:
Q8VHN2
Residue:
1002
Sequence:
MESVRTLWLSVALALAVGSRVVRGHPQPCRVPTRAGASVRLAALLPRAPAARARVLAALATPAPRLPHNLSLELVAVASPTRDPASLARGLCQVLAPPGVVASIAFPEARPELRLLQFLAAATETPVVSVLRREVRTALGAPTPFHLQLDWASPLETILDVLVSLVRAHAWEDIALVLCRVRDPGSLVTLWTNHASQAPKFVLDLSRLDSRNDSLRAGLALLGALEGGGTPVPAAVLLGCSTARAHEVLEAAPPGPQWLLGTPLPAEALPTTGLPPGVLALGETEQHSLEAVVHDMVELVAQALSSMALVHPERALLPAVVNCDDLKTGGSEATGRTLARFLGNTSFQGRTGAVWVTGSSQVHVSRHFKVWSLRRDPLGAPAWATVGSWQDGQLDFQPGAAALRVPSPSGTQARPKLRVVTLVEHPFVFTRESDEDGQCPAGQLCLDPGTNDSARLDALFAALVNGSVPRTLRRCCYGYCIDLLERLAEDLAFDFELYIVGDGKYGALRDGRWTGLVGDLLAGRAHMAVTSFSINSARSQVVDFTSPFFSTSLGIMVRTRDTASPIGAFMWPLHWSMWVGVFAALHLTALFLTLYEWRSPYGLTPRGRNRGTVFSYSSALNLCYAILFGRTVSSKTPKCPTGRFLMNLWAIFCLLVLSSYTANLAAVMVGDKTFEELSGIHDPKLHHPSQGFRFGTVWESSAEAYIKASFPEMHAHMRRHSAPTTPHGVAMLTSDPPKLNAFIMDKSLLDYEVSIDADCKLLTVGKPFAIEGYGIGLPQNSPLTSNLSEFISRYKSSGFIDLLHDKWYKMVPCGKRVFAVTETLQMGVYHFSGLFVLLCLGLGSALLTSLGEHVFYRLVLPRIRRGNKLQYWLHTSQKIHRALNTGPPEGQQERAEQERSGPKDELPATDGAGRWRRVRRAVERERRVRFLLEPGEAGGDRPWLCSNGPGLQAELRELELRIEAARERLRSALLRRGELRALLGDGTRLRPLRLLHAAPAES
  
Inhibitor
Name:
BDBM213889
Synonyms:
5,7-Dichlorokynurenic acid (5,7-DCKA)
Type:
Small organic molecule
Emp. Form.:
C10H5Cl2NO3
Mol. Mass.:
258.058
SMILES:
OC(=O)c1cc(=O)c2c(Cl)cc(Cl)cc2[nH]1
Structure:
Search PDB for entries with ligand similarity: