| Assay Method Information | |
| | Kinase Inhibition Assay |
| Description: | In vitro kinase assay using purified enzyme, was incubated at room temperature with substrate, and test compounds in the presence of 100 uM ATP/ [gamma-33P] ATP. 33P incorporation was measured with a Top Count (Packard). Dose-response profiles were generated, and the IC50 value for inhibition of GSK-3 by the test compound was calculated. |
| Affinity data for this assay | |
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