| Assay Method Information | |
| | Evaluation of Activity to Inhibit Generation of Reactive Oxygen Species and Spore Germination Through NOX Enzyme Inhibition |
| Description: | Specifically, for the evaluation of compound activity, 0.1 mM of the compound was added to a minimum medium (MM20) spore suspension diluted to ⅕. Spores (105/ml) of wild-type red mold strain GZ3639 were inoculated into the medium and cultured at 25° C. for 24 h, followed by microscopic observation. Compounds having a germination inhibition rate and microcycle conidiation to be 50% or more of those in normal germination were selected, and their activities were evaluated at concentrations of 50 µM, 25 µM, and 10 µM. At this time, the activity of substances having a germination inhibition rate of 95% at 10 µM was further evaluated at concentrations of 5 µM, 1 µM, 0.5 µM, and 0.1 µM. Based on the result of this evaluation, the compound concentration (inhibitory concentration 50%, IC50) at which the spore germination inhibition rate was 50% was determined. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |