| Assay Method Information | |
| | Homogeneous Time-resolved Fluorescence (HTRF) Assay |
| Description: | Kinase activity was measured in a homogeneous assay in a 96-well format. Detection was performed by HTRF using an EuK-labelled anti-phospho(S21)-GSK3 alpha and streptavidin-linked XL665 fluorophore which bound to the biotin moiety on the substrate peptide. |
| Affinity data for this assay | |
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