Assay Method Information | |
| Recombinant Aurora A Enzyme Assay |
Description: | In vitro kinase assay using recombinant Aurora A purified from Sf9 cells, was incubated at room temperature with substrate, and test compounds in the presence of 25 uM ATP/ [gamma-33P] ATP. 33P incorporation was measured with a Wallac Beta Counter. IC50 values were calculated using ORIGIN Scientific Graphing Analyses. |
Affinity data for this assay | |
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