Assay Method Information | |
| Phosphatase Inhibition Assay |
Description: | The activity of PTP1B enzyme was assayed with DiFMUP as substrate. Hydrolysis of substrate was monitored on a Victor V plate reader (Wallac). Kinetic measurements were measured using 3-5 concentrations of inhibitor around the IC50 over a range of substrate concentrations (3-500 uM). Data were used to determine Km/Ki/competitive inhibition using GraphPad Prism software. |
Affinity data for this assay | |
---|---|
If you find an error in this entry please send us an E-mail |