| Assay Method Information | |
| | In Vitro FlashPlate Assay |
| Description: | DGAT-1 activity was characterized by catalyzing the transfer of the radiolabeled decanoyl group onto the syn-3 position of didecanoyl glycerol. The resultant radiolabeled tridecanoyl glycerol (tricaprin) preferentially binds to the hydrophobic coating on the phospholipid FlashPlate, and radioactivity was measured in the TopCount Plate Reader. Various concentrations (0.0001 uM to 10 uM) of test compounds were added to individual wells prior to the addition of membranes. The potencies of DGAT-1 inhibition for the compounds were determined by calculating the IC50 values defined as the inhibitor concentration from the sigmoidal dose response curve at which the enzyme activity was inhibited 50%. |
| Affinity data for this assay | |
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