| Assay Method Information | |
| | Determination of Inhibition Constants |
| Description: | The measurements were carried out on a microplate reader. Two concentrations of the substrate and five concentrations of the inhibitor were used. After the addition of the enzyme to reaction mixtures containing chromogenic substrate and test compound, the optical density was monitored at 405 nm. The Ki-values were calculated according to Dixon using a linear regression program. The Ki-values reported are means from at least three determinations. |
| Affinity data for this assay | |
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