| Assay Method Information | |
| | JAK3 Inhibition Assay |
| Description: | An ELISA assay was used to determine the ability of inhibitor to inhibit JAK3 RTK activity. The compounds were incubated with enzyme, 10 uM ATP, and biotinylated peptide substrate. Phosphorylated tyrosine was then detected by sequential incubation with anti-phosphotyrosine antibody. |
| Affinity data for this assay | |
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