Assay Method Information | |
| Radioligand Binding Assay and Luciferase Transcriptional Reporter Gene Assay |
Description: | LXR receptor binding assays were performed in 96-well plates. For each well reaction, GST-LXR-LBD fusion proteins were bound to SPA beads, and radioligand was added in the presence of test compound. The amount of bound ligand was measured on a Packard TopCount using OptiPlates (Packard). IC50s were obtained from dose-response curves. EC50s were generated from Luciferase transcriptional reporter assay in baby hamster kidney cells. |
Affinity data for this assay | |
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