| Assay Method Information | |
| | Homogeneous Time Resolved Fluorescence (HTRF) Assay |
| Description: | Assays were performed in 96-well polystyrene round-bottomed plates in reaction buffer containing biotinylated substrate and purified kinase in the presence of test compounds. Product was detected by adopting a HTRF procedure using streptavidin linked-allophycocyanin and europium-conjugated antiphosphotyrosine antibody. Product was detected using a Victor plate reader (Wallac) with a time delay at 665 nm. |
| Affinity data for this assay | |
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