| Assay Method Information | |
| | Enzyme Inhibition Assay |
| Description: | Compounds were tested for inhibition of sirtuin (SIRT1 or SIRT2) using the human recombinant enzyme provided with the Fluor de Lys fluorescence-based assay kit (BIOMOL) in a 96-well microplate format. Positive controls were provided by the known inhibitors tenovin-6 and cambinol. After incubation, plates were read in a Spectra max Gemini XS fluorimeter with an excitation wavelength of 355 nm and an emission wavelength of 460 nm. IC50 values were determined for compounds that had over 60% inhibition at 60 uM (repeated at least twice). |
| Affinity data for this assay | |
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