| Assay Method Information | |
| | PDE4A Inhibition Assay |
| Description: | Inhibition of PDE4A was performed using IMAP technology (Molecular Devices, CA). PDE4A1A mixture was dispensed into 1536-well black/solid bottom assay plates (Greiner Bio-One North America, NC) using a Flying Reagent Dispenser (Aurora Discovery, CA). The plates were centrifuged at 1000 rpm for 30s and compound was transferred to the assay plate using a Kalypsys pin tool. After incubation, cAMP was dispensed into the plates to initiate the reaction. IMAP binding reagent was then added to the assay plate after the reaction. The fluorescence polarization (FP) signal (Ex=485 nm, Em= 530 nm) was measured on Viewlux plate reader (Perkin Elmer, MA). |
| Affinity data for this assay | |
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