| Assay Method Information | |
| | sPLA2-V activity assay |
| Description: | Substrate 1,2-bis(heptanoylthio)-glycerophosphocholine and human recombinant PLA2-V were resuspended in assay buffer, and DTNB was dissolved in an aqueous solution of Tris-HCl (pH 8). Enzyme and DTNB yielded final concentrations of 100 ng/mL and 87 µM. The assay was progressed in 96-well microliter plates containing DTNB, substrate solution, and the respective test substance in an aqueous buffer solution (pH 7.5) containing 94 mM KCl, 9 mM CaCl2, 24 mM Tris, and 280 µM Triton-X 100 at room temperature. |
| Affinity data for this assay | |
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