| Assay Method Information | |
| | ADP-Glo Kinase Assay |
| Description: | Some compounds of formula (I) described herein were assayed in vitro for their ability to inhibit the lipid kinase activity of PI3Ks, by using a non-radioactive method.In details, His and GST-tagged recombinant proteins (purchased from JenaBioscience Germany; PI3Kalpha #PR-335, PI3Kbeta #PR-344, PI3Kgamma #PR-343, PI3Kdelta #PR-345) for each isoform PI3Kalpha , beta , gamma and delta, were incubated with 10 uM of ATP, with lipid micells containg the appropriate substrate phosphatidylinositol and the phosphatidilserine, and different concentrations of the formula (I) compounds.After 30 minutes of incubation at room temperature, the amount of ADP produced from ATP following the activation of kinase was measured with a luminescent kinase assay, ADP-Glo Kinase Assay (purchased from Promega USA #V9101).The luminescent signal positively correlates with the formed ADP and, therefore, with the kinase activity. |
| Affinity data for this assay | |
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