| Assay Method Information | |
| | Thymidine Phosphorylase Assay |
| Description: | The Thymidine phosphorylase/PD-ECGF (E. coli) activity was determined by measuring the absorbance at 290 nm spectrophotometrically. In brief, total reaction mixture of 200 uL contained 145 uL of potassium phosphate buffer (pH 7.4), 30 uL of enzyme (E. coli) at concentration 0.05 and 0.002 U, respectively, were incubated with 5 uL of test materials for 10 min at 25 °C in microplate reader. After incubation, preread at 290 nm was taken to deduce the absorbance of substrate particles. Substrate (20 uL, 1.5 mM), was dissolved in potassium phosphate buffer, was immediately added to plate and continuously read after 10, 20, and 30 min in microplate reader (spectra max, molecular devices, CA, USA). All assays were performed in triplicate. |
| Affinity data for this assay | |
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