| Assay Method Information | |
| | 5α-R1 Activity Assay |
| Description: | The activity of the 5α-R type 1 isozyme was determined by following the conversion of T to DHT at pH 7.5. 2 nM of [1,2,6,7 3H] T, and different concentrations of unlabeled T (5 × 10^-7-6.3 × 10^-6 M) or DHT (5 × 10^-7-2.7 × 10^-5M) and 2 mM NADPH11 was prepared. The reaction in duplicate was started when 5α-R type 1 isozyme fraction (60 μg protein in a volume of 7.5 μL) was added to the mixtureand incubated at 37°C for 60 min. |
| Affinity data for this assay | |
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