| Assay Method Information | |
| | Binding Assay |
| Description: | The binding properties of the test compounds on adenosine receptors were determined in binding studies with radioligands. For this purpose, membrane preparations of the human adenosine receptor subtypes were produced from cell lines having recombinant receptor expression (CHO cells for the A1 receptor, HEK293 cells for the A2a, A2b and A3 receptors). The following radioligands were used in the experiments: [3H]-DPCPX for the A1 receptor, [3H]-CGS 21680 for the A2a receptor, [3H]-CPX for the A2b receptor and [125I]-AB-MECA for the A3 receptor. The test substances were each tested in 8 different concentrations and 2 repeat tests per concentration. The displacement of the particular radioligand by the test compound was expressed as percentage inhibition of the specific binding of the controls. |
| Affinity data for this assay | |
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