| Assay Method Information | |
| | Jurkat HIV Latency assay |
| Description: | For the Jurkat HIV Latency assay, compounds are dissolved and titrated in DMSO and diluted 100-fold in assay medium (RPMI-1640 containing 10% fetal bovine serum) containing an equal mixture of three HIV-infected Jurkat cell clones (C16, I15 and N6) at a total concentration of 1-2×10e5 cells/mL. To test stability, compounds are pre-incubated in an assay medium for 48 hours at 37° C. prior to adding cells (48 hr EC50). Compounds that induce HIV expression result in a dose-dependent production of the HIV expressed luciferase enzyme. After the incubation of cells with compound for 24 hours at 37° C., HIV activation and cytotoxicity are determined by measuring luminescence after the addition of Promega Steady-Glo Luciferase Assay reagent or CellTiter-Glo Luminescent Cell Viability Assay reagent, respectively. |
| Affinity data for this assay | |
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