| Assay Method Information | |
| | Axl Enzyme Inhibition Assay |
| Description: | Axl was assayed using a fluorescence polarization (FP) competition immunoassay that measured Axl phosphorylation of polyGlu4Tyr. The reaction was initiated with ATP, incubated 11 min at room temperature, and quenched by the addition of EDTA. The FP buffer/tracer/phospho-Y antibody mix (Invitrogen) was added to the quenched reaction, and FP was measured using an Analyst reader (Molecular Devices) at excitation/emission of 485/530 nm. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |