| Assay Method Information | |
| | ACAT-1 Enzyme Inhibition Assay |
| Description: | The assay mixture contains test compound, membrane protein, cholesterol solubilized in Triton WR-1339, and glutathione in a 96-deep well plate. The reaction was started by adding substrate [14C]oleoyl coenzyme A and incubated. After the reaction was stopped, radioactive cholesteryl oleate product was separated into the organic heptane phase. ACAT-1 activity was quantified by counting aliquots of the upper heptane layer by liquid scintillography. |
| Affinity data for this assay | |
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