| Assay Method Information | |
| | Surface Plasmon Resonance (SPR) |
| Description: | SPR measurements were performed with a Biacore X100 instrument (GE Healthcare Life Sciences, Piscataway, NJ) at 15.0 °C. The biotinylated PTHRct-22 ligand was immobilized on an SA streptavidin sensor chip (GE Healthcare Life Sciences) usingthe manufacturer's protocol until the desired response target was reached. PDZ1 or PDZ2 analyte, dissolved in 10 mM HEPES buffer (pH 7.4), 150 mM NaCl, 3 mM EDTA, and 0.005% surfactant polysorbate 20, was injected on the PTHRct-coated sensor surface at increasing concentrations from 10 nM to 100 μM. At the end of each ligand injection−dissociation cycle, the sensor chip was regenerated with 4.0 M MgCl2, 50 mM triethylamine (pH 9.15), and HBS-EP buffer. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |