Assay Method Information | |
| ChEMBL_2251914 (CHEMBL5166124) |
Description: | Inhibition of human recombinant N-terminal GST-tagged ERK5 (1 to 398 residues) expressed in Escherichia coli using biotinylated peptide Ahx-PPGDYSTTPGGTLFSTTPGGTRI as substrate preincubated for 15 mins followed by substrate addition measured after 60 mins in presence of ATP by TR-FRET method |
Affinity data for this assay | |
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