| Assay Method Information | |
| | Isothermal Titration Calorimetry (ITC) |
| Description: | ITC measurements were conducted on a Microcal iTC200 (Malvern Instruments) in buffer consisting of 25 mM Tris (pH 8.0) and 100 mM NaCl. Proteins were exchanged into ITC buffer by gel filtration using a Superdex200 column. Wild-type modified synthetic peptides were purchased from Genscript and are described in Results. One millimolar PTHR peptides (wild-type and mutant variants) were titrated into 100 μM individual proteins, PDZ1 or PDZ2, at 25 °C. |
| Affinity data for this assay | |
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