Assay Method Information | |
| ChEMBL_1523832 (CHEMBL3632393) |
Description: | Inhibition of GARFTase in human KB cells expressing FRalpha/PCFT/RFC assessed as incorporation of [14C(U)]-glycine into [14C]-formyl GAR preincubated for 1 hr followed by azaserine, L-glutamine, 14C(U)]-glycine addition measured after 16 hrs |
Affinity data for this assay | |
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