The application of HPLC with on-line coupled UV/MS-biochemical detection for isolation of an acetylcholinesterase inhibitor from narcissus 'Sir Winston Churchill'

K Ingkaninan; A Hazekamp; C M de Best; H Irth; U R Tjaden; R van der Heijden; J van der Greef; R Verpoorte

doi:10.1021/np9905719

The application of HPLC with on-line coupled UV/MS-biochemical detection for isolation of an acetylcholinesterase inhibitor from narcissus 'Sir Winston Churchill'

J Nat Prod. 2000 Jun;63(6):803-6. doi: 10.1021/np9905719.

Authors

K Ingkaninan¹, A Hazekamp, C M de Best, H Irth, U R Tjaden, R van der Heijden, J van der Greef, R Verpoorte

Affiliation

¹ Division of Pharmacognosy, Leiden/Amsterdam Center for Drug Research, Gorlaeus Laboratories, Leiden University, P.O. Box 9502, 2300 RA, Leiden, The Netherlands.

PMID: 10869205
DOI: 10.1021/np9905719

Abstract

An HPLC with on-line coupled UV/MS-biochemical detection method for acetylcholinesterase (AChE) inhibitors in natural sources has been developed. The potential of this method is shown by the isolation of a new AChE inhibitor from the alcoholic extract of Narcissus 'Sir Winston Churchill'. Combining a prefractionation technique using centrifugal partition chromatography with the on-line HPLC-UV/MS-biochemical detection resulted in the isolation of the active compound that was identified as ungiminorine. This alkaloid shows a mild inhibitory effect on AChE.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alkaloids / chemistry
Alkaloids / isolation & purification*
Amaryllidaceae Alkaloids
Cholinesterase Inhibitors / isolation & purification*
Chromatography, High Pressure Liquid / methods*
Galantamine / chemistry
Magnetic Resonance Spectroscopy
Mass Spectrometry
Plants / chemistry*
Spectrophotometry, Ultraviolet

Substances

Alkaloids
Amaryllidaceae Alkaloids
Cholinesterase Inhibitors
ungiminorine
Galantamine