Affinity DataKi: 1.58E+4nM ΔG°: -27.4kJ/mole IC50: 2.10E+4nMpH: 7.4 T: 2°CAssay Description:CA activity was assayed according to method of Verpoorte et al. [Verpoorte et al., J. Biol. Chem., 242:4221-4229] described previously by Innocenti e...More data for this Ligand-Target Pair
Affinity DataKi: 1.73E+4nM IC50: 2.13E+4nMpH: 8.0Assay Description:Enzymatic activity was measured by Beutler's method with a Shimadzu Spectrophotometer UV-(1208), at 25°C. The assay system contained 100 mM Tris-...More data for this Ligand-Target Pair
Affinity DataKi: 2.85E+4nM ΔG°: -25.9kJ/mole IC50: 2.71E+4nMpH: 7.4 T: 2°CAssay Description:CA activity was assayed according to method of Verpoorte et al. [Verpoorte et al., J. Biol. Chem., 242:4221-4229] described previously by Innocenti e...More data for this Ligand-Target Pair
Affinity DataIC50: 3.56E+4nMAssay Description:Inhibition of human MRP4 overexpressed in Sf9 cell membrane vesicles assessed as uptake of [3H]-estradiol-17beta-D-glucuronide in presence of ATP and...More data for this Ligand-Target Pair
Affinity DataKi: 3.41E+4nM IC50: 4.86E+4nMpH: 7.0Assay Description:Glutathione peroxidase was assayed in a l-ml system containing 0.1 M potassium phosphate buffer, pH 7.0, 0.2 mM NADPH, 1 i.u. glutathione reductase, ...More data for this Ligand-Target Pair
Affinity DataKi: 3.54E+4nM IC50: 6.18E+4nMpH: 6.5Assay Description:Enzymatic activity was determined spectrophotometrically by measuring the conjugation of CDNB with GSH. The 1 ml assay mixture contained 0.5 mM CDNB,...More data for this Ligand-Target Pair
Affinity DataIC50: >1.33E+5nMAssay Description:Inhibition of human MRP2 overexpressed in Sf9 cell membrane vesicles assessed as uptake of [3H]-estradiol-17beta-D-glucuronide in presence of ATP and...More data for this Ligand-Target Pair
Affinity DataIC50: >1.33E+5nMAssay Description:Inhibition of human MRP3 overexpressed in Sf9 insect cell membrane vesicles assessed as uptake of [3H]-estradiol-17beta-D-glucuronide in presence of ...More data for this Ligand-Target Pair
Affinity DataIC50: >1.33E+5nMAssay Description:Inhibition of human BSEP overexpressed in Sf9 cell membrane vesicles assessed as uptake of [3H]-taurocholate in presence of ATP measured after 15 to ...More data for this Ligand-Target Pair