BindingDB logo
myBDB logout
Solution Informationhelp
Enzyme: Mu-type opioid receptor
inhibitor: BDBM54816
substrate: n/a
 
Solution Type: Aqueous
pH at Preparation: n/a
Temp. Prep.: n/a
Comments: Assay Materials: 1) 384-well plates, black with clear bottom (Greiner# 781091) 2) U2OS (Human Osteosarcoma) cell line stably expressing the Beta-arrestin GFP and the MOR1 receptor 3) Culture Media: MEM with L-glutamine, Pen-strep, 10% Fetal Bovine Serum and selection antibiotics - 200 ug/ml G418 and 100 ug/ml Zeocin 4) Positive Control Working Solution: Damgo (Tocris Cookson #1157 - 5mM stock in DMSO) diluted in water to 25 uM. 5) Negative Control Working Solution: 100% DMSO 6) Test compounds from dry powder working solution: 10 mM in 100% DMSO 7) Fixative Working Solution: 6% Paraformaldehyde (PFA) diluted in PBS. 8) Nuclear Stain Working Solution: DAPI (Invitrogen, D1306) diluted to 150 ng/ml in DAPI buffer (10 mM TRIS, 10 mM EDTA, 100 mM NaCl, pH 7.4). Assay Procedure: 1) 45 ul of cell suspension (200,000 cells/ml in culture medium) was dispensed in each well of the assay plates using a Wellmate bulk dispenser. 2) Plates are incubated overnight or approximately 20 hours at 37
 
 

If you find an error in this entry please send us an E-mail
   
    

Home

|

Search

|

Deposit

|

SiteMap

|

About us

|

Email us

|

Info

 
Last update November 1, 2007
©2000 BindingDB. All rights reserved.