Cell Reactant:
HIV-1 Nef
Syringe Reactant:
BDBM22592
Meas. Tech.:
Isothermal Titration Calorimetry
Entry Date.:
06/14/08
ΔG°:
-33.3±n/a (kJ/mole)
pH:
7±n/a
Log10Kb:
5.5
Temperature:
298.15±n/a (K)
ΔHobs :
-2.3±0.17 (kJ/mole)
Corrected for ΔHioniz:
no
Stoich. Param.:
1.2
ΔS° :
0.104±n/a (kJ/mole-K)
Citation
 Betzi, SRestouin, AOpi, SArold, STParrot, IGuerlesquin, FMorelli, XCollette, Y Protein protein interaction inhibition (2P2I) combining high throughput and virtual screening: Application to the HIV-1 Nef protein. Proc Natl Acad Sci U S A 104:19256-61 (2007) [PubMed]  Article
Cell React
Source:
The HIV-1 Nef core domain (residues 57-205) was expressed in Escherichia coli as glutathione S-transferase fusion proteins.
Name:
Protein Nef
Synonyms:
3ORF | F-protein | HIV-1 Nef | NEF_HV1BR | Negative factor | Protein Nef | nef
Type:
C-terminal core protein
Mol. Mass.:
23340.47
Organism:
Human immunodeficiency virus type 1 (isolate BRU/LAI group M subtype B)
Description:
P03406
Residue:
206
Sequence:
MGGKWSKSSVVGWPTVRERMRRAEPAADGVGAASRDLEKHGAITSSNTAATNAACAWLEAQEEEEVGFPVTPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIHSQRRQDILDLWIYHTQGYFPDWQNYTPGPGVRYPLTFGWCYKLVPVEPDKVEEANKGENTSLLHPVSLHGMDDPEREVLEWRFDSRLAFHHVARELHPEYFKNC
  
Syringe React
Name:
BDBM22592
Synonyms:
1-carboxy-7-(dimethylamino)-4-hydroxy-3-oxo-3H-phenoxazin-10-ium chloride | Compound D12 | gallocyanine
Type:
Coloring Agent, DNA-interacting Compound
Emp. Form.:
C15H13N2O5
Mol. Mass.:
301.2736
SMILES:
CN(C)c1ccc2[nH+]c3c(cc(=O)c(O)c3oc2c1)C(O)=O
Structure:
Search PDB for entries with ligand similarity: