Cell Reactant:
ferricytochrome c peroxidase (CcP)
Syringe Reactant:
iso-1-cytochrome c (Cc)
Meas. Tech.:
ITC
Entry Date.:
12/15/00
ΔG°:
-34.02±0.42 (kJ/mole)
pH:
6±n/a
Temperature:
298.15±n/a (K)
ΔH° :
-32.34±1.26 (kJ/mole)
ΔHobs :
-26.04±n/a (kJ/mole)
Corrected for ΔHioniz:
yes
Protons Released:
0.6
ΔCp :
-0.9072±0.2604 (kJ/mole)
Stoich. Param.:
1
ΔS° :
0.0268±0.006 (kJ/mole-K)
Comments:
test the effect of pH on binding
Citation
 Wang, XPielak, GJ Equilibrium thermodynamics of a physiologically-relevant heme-protein complex. Biochemistry 38:16876-81 (1999) [PubMed]  Article
Cell React
Source:
recombinant yeast CcP was expressed and purified from E.coli
Prep. Method:
CcP and Cc were dialyzed (Slide -A_Lyzer membrane) against buffer in the same beaker for > 36 h with three buffer changes
Name:
ferricytochrome c peroxidase (CcP)
Synonyms:
n/a
Type:
Enzyme
Mol. Mass.:
40387.06
Organism:
Saccharomyces cerevisiae
Description:
A0A0D3U836
Residue:
362
Sequence:
MTTAVRLLPSLGRTAHKRSLYLFSAAAAAAAAAATFAYSQSHKRSSSSPGGGSNHGWNNWGKAAALASTTPLVHVASVEKGRSYEDFQKVYNAIALKLREDDEYDNYIGYGPVLVRLAWHISGTWDKHDNTGGSYGGTYRFKKEFNDPSNAGLQNGFKFLEPIHKEFPWISSGDLFSLGGVTAVQEMQGPKIPWRCGRVDTPEDTTPDNGRLPDADKDAGYVRTFFQRLNMNDREVVALMGAHALGKTHLKNSGYEGPWGAANNVFTNEFYLNLLNEDWKLEKNDANNEQWDSKSGYMMLPTDYSLIQDPKYLSIVKEYANDQDKFFKDFSKAFEKLLENGITFPKDAPSPFIFKTLEEQGL
  
Syringe React
Source:
purified from yeast
Prep. Method:
CcP and Cc were dialyzed (Slide -A_Lyzer membrane) against buffer in the same beaker for > 36 h with three buffer changes
Name:
iso-1-cytochrome c (Cc)
Synonyms:
n/a
Type:
Protein
Mol. Mass.:
12191.93
Organism:
Saccharomyces cerevisiae
Description:
A0A0C6AZG8
Residue:
109
Sequence:
MTEFKAGSAKKGATLFKTRCLQCHTVEKGGPHKVGPNLHGIFGRHSGQAEGYSYTDANIKKNVLWDENNMSEYLTNPKKYIPGTKMAFGGLKKEKDRNDLITYLKKACE