Cell Reactant:
Grb2-SH2
Syringe Reactant:
pY317 V(+3)A
Meas. Tech.:
ITC
Entry Date.:
11/29/00
ΔG°:
-34.31±0.336 (kJ/mole)
pH:
7.5±n/a
Temperature:
293.15±n/a (K)
ΔH° :
-25.07±0.8 (kJ/mole)
ΔHobs :
-25.07±n/a (kJ/mole)
Corrected for ΔHioniz:
yes
Protons Released:
0
ΔS° :
0.032±0.004 (kJ/mole-K)
Citation
 McNemar, CSnow, MEWindsor, WTProngay, AMui, PZhang, RDurkin, JLe, HVWeber, PC Thermodynamic and structural analysis of phosphotyrosine polypeptide binding to Grb2-SH2. Biochemistry 36:10006-14 (1997) [PubMed]  Article
Cell React
Source:
DNA construct for the Grb2-SH2 domain was expressed in E.coli
Purity:
>98%
Prep. Method:
Mono-Q and Mono-S columns
Name:
Growth factor receptor-bound protein 2
Synonyms:
GRB2 | GRB2 adapter protein | Grb2-SH2 | Growth factor receptor-bound protein 2
Type:
Protein
Mol. Mass.:
25205.04
Organism:
Homo sapiens (Human)
Description:
P62993
Residue:
217
Sequence:
MEAIAKYDFKATADDELSFKRGDILKVLNEECDQNWYKAELNGKDGFIPKNYIEMKPHPWFFGKIPRAKAEEMLSKQRHDGAFLIRESESAPGDFSLSVKFGNDVQHFKVLRDGAGKYFLWVVKFNSLNELVDYHRSTSVSRNQQIFLRDIEQVPQQPTYVQALFDFDPQEDGELGFRRGDFIHVMDNSDPNWWKGACHGQTGMFPRNYVTPVNRNV
  
Syringe React
Source:
AnaSpec Inc. (San Jose, CA)
Prep. Method:
Fmoc solid-phase peptide synthesis
Name:
pY317 V(+3)A
Synonyms:
n/a
Type:
Peptide
Mol. Mass.:
680.71
Organism:
n/a
Description:
alanine substitution of pY317, a phosphotyrosine polypeptide
Residue:
6
Sequence:
SYVNAQ