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Target
E3 ubiquitin-protein ligase Mdm2
Ligand
BDBM31219
Substrate
6-FAM labeled octapeptide
Meas. Tech.
Fluorescence Polarization (FP) Binding Assay
pH
7.4±n/a
Temperature
296.15±n/a K
Ki
10000±n/a nM
Citation
 Zhang, R; Mayhood, T; Lipari, P; Wang, Y; Durkin, J; Syto, R; Gesell, J; McNemar, C; Windsor, W Fluorescence polarization assay and inhibitor design for MDM2/p53 interaction. Anal Biochem 331:138-46 (2004) [PubMed]  Article 
Target
Name:
E3 ubiquitin-protein ligase Mdm2
Synonyms:
Double minute 2 protein | Double minute 2 protein (HDM2) | E3 ubiquitin-protein ligase Mdm2 (p53-binding protein Mdm2) | Hdm2 | Human Double Minute 2 (HDM2) | MDM2 | MDM2-MDMX | MDM2_HUMAN | p53-Binding Protein MDM2 | p53-binding protein
Type:
Oncoprotein
Mol. Mass.:
55196.54
Organism:
Homo sapiens (Human)
Description:
Q00987
Residue:
491
Sequence:
MCNTNMSVPTDGAVTTSQIPASEQETLVRPKPLLLKLLKSVGAQKDTYTMKEVLFYLGQYIMTKRLYDEKQQHIVYCSNDLLGDLFGVPSFSVKEHRKIYTMIYRNLVVVNQQESSDSGTSVSENRCHLEGGSDQKDLVQELQEEKPSSSHLVSRPSTSSRRRAISETEENSDELSGERQRKRHKSDSISLSFDESLALCVIREICCERSSSSESTGTPSNPDLDAGVSEHSGDWLDQDSVSDQFSVEFEVESLDSEDYSLSEEGQELSDEDDEVYQVTVYQAGESDTDSFEEDPEISLADYWKCTSCNEMNPPLPSHCNRCWALRENWLPEDKGKDKGEISEKAKLENSTQAEEGFDVPDCKKTIVNDSRESCVEENDDKITQASQSQESEDYSQPSTSSSIIYSSQEDVKEFEREETQDKEESVESSLPLNAIEPCVICQGRPKNGCIVHGKTGHLMACFTCAKKLKKRNKPCPVCRQPIQMIVLTYFP
  
Inhibitor
Name:
BDBM31219
Synonyms:
2-phenoxybenzoyltryptophan derivative, R4C1
Type:
Small organic molecule
Emp. Form.:
C25H19N3O4
Mol. Mass.:
425.4361
SMILES:
OC(=O)[C@H](Cc1c[nH]c2ccc(cc12)C#N)NC(=O)c1ccccc1Oc1ccccc1 |r|
Structure:
Search PDB for entries with ligand similarity:
Substrate
Name:
6-FAM labeled octapeptide
Synonyms:
n/a
Type:
fluorescent peptide
Mol. Mass.:
2832.21
Organism:
n/a
Description:
Fluorophore 6-carboxyfluorescein was covalently attached to the side chain amino group of the Dpr residue in the peptide.
Residue:
26
Sequence:
ACPHEARGDPRACCRTRPGLGLLENH