Target
Genome polyprotein [1424-1463]/[1502-1685]
Ligand
BDBM33250
Substrate
BDBM33248
Meas. Tech.
Enzyme Inhibition Assay (IC50) and Fluorescence Quench Assay (KD)
pH
7.5±n/a
Temperature
310.15±n/a K
IC50
24500±n/a nM
Kd
21000±n/a nM
Comments
Hill slope=3.5.
Citation
 Bodenreider, CBeer, DKeller, THSonntag, SWen, DYap, LYau, YHShochat, SGHuang, DZhou, TCaflisch, ASu, XCOzawa, KOtting, GVasudevan, SGLescar, JLim, SP A fluorescence quenching assay to discriminate between specific and nonspecific inhibitors of dengue virus protease. Anal Biochem 395:195-204 (2009) [PubMed]  Article 
Target
Name:
Genome polyprotein [1424-1463]/[1502-1685]
Synonyms:
WNV CF40.Gly.NS3Pro | WNV NS2B(H):NS3 | West Nile Virus (WNV) NS2B-NS3 Protease
Type:
Cofactor/Enzyme
Mol. Mass.:
n/a
Description:
It is the catalytically active recombinant WNV protease, consisting of a 40-residue component of cofactor NS2B tethered via a nonapeptide (G4SG4) to the N-terminal 184 residues of NS3.
Components:
This complex has 2 components.
Component 1
Name:
Genome polyprotein [1424-1463]
Synonyms:
Flavivirin protease NS2B regulatory subunit | NS2B | POLG_WNV
Type:
Cofactor Domain
Mol. Mass.:
4431.59
Organism:
West Nile virus (WNV)
Description:
P06935[1424-1463]
Residue:
40
Sequence:
IERTADITWESDAEITGSSERVDVRLDDDGNFQLMNDPGA
  
Component 2
Name:
Genome polyprotein [1502-1685]
Synonyms:
Flavivirin protease NS3 catalytic subunit | NS3 | POLG_WNV
Type:
Catalytic Subunit
Mol. Mass.:
19839.27
Organism:
West Nile virus (WNV)
Description:
P06935[1502-1685]
Residue:
184
Sequence:
GGVLWDTPSPKEYKKGDTTTGVYRIMTRGLLGSYQAGAGVMVEGVFHTLWHTTKGAALMSGEGRLDPYWGSVKEDRLCYGGPWKLQHKWNGHDEVQMIVVEPGKNVKNVQTKPGVFKTPEGEIGAVTLDYPTGTSGSPIVDKNGDVIGLYGNGVIMPNGSYISAIVQGERMEEPAPAGFEPEML
  
Inhibitor
Name:
BDBM33250
Synonyms:
phthalazine-based compound, 1
Type:
Small organic molecule
Emp. Form.:
C28H28N8
Mol. Mass.:
476.5755
SMILES:
CC1CNC(=N1)c1ccc(Nc2nnc(Nc3ccc(cc3)C3=NC(C)CN3)c3ccccc23)cc1 |c:4,t:24|
Structure:
Search PDB for entries with ligand similarity:
Substrate
Name:
BDBM33248
Synonyms:
Bz-Nle-Lys-Arg-Arg-AMC | Bz-nKRR-AMC | benzoyl-Nle-Lys-Arg-Arg-7-amido-4-methylcoumarin
Type:
fluorogenic tetrapeptide substrate
Emp. Form.:
n/a
Mol. Mass.:
n/a
SMILES:
n/a
Structure: