Assay Method Information

Assay Name:  Aromatase Assay
Description:  The enzyme activity was assayed by measuring the 3H-labeled H2O formed from [1beta-3H] Androstenedione during aromatization. After incubation, the reaction was terminated by CHCl3 extraction and centrifugation. Aliquots of the water phase were assayed for the presence of 3H2O by counting using a liquid scintillation spectrometer.
Affinity data for this assay
 

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