| Assay Method Information | |
| | Caspase Inhibition Assay |
| Description: | The substrate peptides terminating in AMC are processed by caspases with or without inhibitors. The amount of AMC released was determined by using a Victor microplate fluorometer (Perkin-Elmer Life Sciences, Boston, MA) at excitation and emission wavelengths of 360 and 460 nm. Compounds were tested at a single dose (50 uM), and full IC50 curves were run for all compounds expressing measurable inhibitory activity. Results were expressed as mean values of three independent measurements. |
| Affinity data for this assay | |
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