| Assay Method Information | |
| | Enzyme Inhibition Assay |
| Description: | The cholinesterase assays were performed using colorimetric method reported by Ellman. Enzyme activity was determined by measuring the absorbance at 405 nm. The activities were compared to the activity of a drug-free control and expressed as a percent of activity. IC50 values were determined at 0.2 mM total substrate concentration from a plot of percent enzyme activity versus -log[I], where [I] refers to inhibitor concentration. The presented values are the averages of three determinations. |
| Affinity data for this assay | |
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