Assay Method Information

Assay Name:  Caspase Inhibition Assay
Description:  The substrate peptides terminating in AMC are processed by caspases with or without inhibitors, and the accumulation of AMC was assessed with a Cytofluor fluorescent plate reader at excitation and emission wavelengths of 380 and 460 nm. Compounds were tested, and full IC50 curves were run for all compounds expressing measurable inhibitory activity.
Affinity data for this assay
 

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