Assay Method Information | |
| SARS-CoV 3CL Protease Inhibition Assay |
Description: | The effects of compound on enzyme activity were measured by using a fluorogenic peptide cleavage assay. Enhanced fluorescence caused by cleavage of the substrate peptide was monitored at 538 nm with excitation at 355 nm. The initial velocities of the inhibited reactions of 50 nM SARS 3CL protease and 6 uM fluorogenic substrate were plotted against the different inhibitor concentrations to obtain the IC50. |
Affinity data for this assay | |
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