| Assay Method Information | |
| | Thallium Flux Assay |
| Description: | Assay Protocol: The ROMK channel functional thallium flux assay was performed in 384 wells, using the FLIPR-Tetra instrument. HEK-hKir1.1 cells were seeded in Poly-D-Lysine microplates and kept in a 37° C.-10% CO2 incubator overnight. On the day of the experiment, the growth media was replaced with the FluxOR reagent loading buffer and incubated, protected from light, at ambient temperature (23-25° C.) for 90 min. The loading buffer was replaced with assay bufferątest compound followed by 30 min incubation at ambient temperature, where the thallium/potassium stimulant was added to the microplate. |
| Affinity data for this assay | |
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