Assay Method Information | |
| Scintillation Proximity Assay (SPA) |
Description: | SPA uses 125I as energy donor and scintillant-coated beads as energy acceptor. The labeled ligand is captured by the biotinylated Src-SH2 protein immobilized on streptavidin beads, resulting in the emission of light. IC50 values were obtained from competitive binding assay. |
Affinity data for this assay | |
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