Assay Method Information

Assay Name:  IMPDH2 Enzyme Inhibition and Human T-Lymphoblast (CEM) Proliferation Inhibition Assays
Description:  Inhibition assays were performed using recombinant human IMPDH type 2 and varying concentrations of inhibitor. The production of NADH was monitored by following changes in absorbance at 340 nm. The concentrations of compound required to inhibit NADH accumulation by 50% (IC50) were calculated using a four-parameter logistic plot. The in vivo activities of compounds were also evaluated the inhibition of T-cell proliferation in CEM lymphoblast cells. The EC50/IC50 values were calculated using a four-parameter logistic plot.
Affinity data for this assay
 

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