Assay Method Information

Assay Name:  Enzyme Inhibition Assay
Description:  The recombinant human cathepsin enzyme was preincubated with inhibitor for 20 minutes prior to addition of substrate. The substrate hydrolysis was monitored by the increase in fluorescence at an excitation wavelength of 380nm and an emission wavelength of 450 nm on a Gemini EM fluorometer. The reaction progress curve was fitted to the Morrison equation using PlateKi (BioKin) and the apparent inhibition constant was converted to the inhibition constant (Ki) for competitive inhibitors.
Affinity data for this assay
 

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